Benchtop Flow Cytometers
BD LSR (referred to as LSRII) S/N H1014
BD LSRFortessa (referred to as Fortessa) S/N H5B500011
The LSRII and Fortessa are used for sophisticated analysis of surface, intracellular and nuclear proteins on diverse cell types. Both systems are equipped with four lasers and can detect the following 16 fluorophores (FITC, PerCP or PerCPCy5.5, PE, PE-TxRed, PECy5, PECy5.5, PECy7, Pacific Blue, AmCyan OR V500, BV or Qdot565, BV or Qdot605, BV or Qdot655, BV 710, AF700, APC OR AF647, and APC-Cy7). The LSRII has the hardware for automated data acquisition from 96 well plates (High Throughput Sampler).
Benchtop cytomters – Fortessa, LSR2 – Instrument Information
PA VA: 3801 Miranda Ave, Palo Alto
Bldg.101, 4th Floor, Rm. B4-115
Primary Contact: Lusijah Sutherland
Cost to use:
There is no direct cost to individual users or labs. The facility is supported in its entirety by PAVIR except for the cost of supplies which is split among the laboratories proportional to their use, which averages out to $2-3/hr.
Written strategies for troubleshooting are posted above the instrument. In addition, there are several experienced users in the research area who can help troubleshoot or answer questions.