Flow Cytometry Core – Flow Cytometer Information

Flow Cytometer Information

  • Availability: 24 hours, 7 days a week for verified users.
  • Normal Working Hours with Assistance and Flow Cytometer Set Up: Monday- Friday 7:30am-4pm.

Brandon is accessible by phone from 7:30AM to 9PM, Monday through Thursday, and Friday 7:30AM to 4PM unless on PTO. Calls after 9PM or on the weekends will be sent to voice mail. I will address voice mails during off hours as able but most often not until the next business day.

All benchtop BD Flow Cytometers are located at the address below:

Palo Alto VA
3801 Miranda Ave, Palo Alto
Bldg.101, 4th Floor, Rm. B4-115

Contact for training and/or access to the flow cytometers
Primary Contact: Brandon Carter.
Phone: (650) 223-1657
Email: bcarter@pavir.org


LSRII (Nickname: Nightcrawler)

BD Serial # S/N H1014

LSR2-Night-Crawler

The LSRII is a flow cytometer equipped with four lasers (405nm (violet), 488nm (blue), 532nm (yellow-green) and 633nm (red)) and can detect up to 16 fluorophores:

Common fluorophores used in this configuration:

Violet (405nm) Laser (6 detectors):   Pacific Blue, AmCyan OR V500, BV605 or Qdot605, BV or Qdot655, BV710, BV786

Blue (488nm) Laser (4 detectors): FSC, SSC, FITC, PerCP or PerCPCy5.5

Yellow/Green (532nm) Laser (5 detectors):  PE, PE-TexasRed, PE-Cy5, PE-Cy5.5, PECy7

Red (635nm) Laser (3 detectors): AF700, APC or AF647, and APC-Cy7

The LSRII has the hardware for automated data acquisition from 96 well plates as well (High Throughput Sampler).

Follow link to see detailed LSRII (Nightcrawler) lasers and filter configurations.

Follow link for assistance building an experimental panel for use on the LSRII (NightCrawler) using FluoroFinder: https://app.fluorofinder.com/pavir


LSRFortessa (Nickname: Storm)

BD Serial # H5B500011

LSRFortessa-Storm

The LSRFortessa is a flow cytometer equipped with five lasers (355nm (near UV), 405nm (violet), 488nm (blue), 532nm (yellow-green) and 640nm (red)) and can detect up to 18 fluorophores:

Common fluorophores used in this configuration:

UV (355nm) laser (3 detectors):  BUV395, BUV450, BUV737

Violet (405 nm) laser (6 detectors): Pacific Blue, AmCyan or V500, BV or Qdot605, BV or Qdot655, BV710, BV786

Blue (488nm) laser (4 detectors): FSC, SSC, FITC, PerCP or PerCPCy5.5

Yellow/Green (532nm) laser (4 detectors): PE, PE-TexasRed, PE-Cy5.5, PE-Cy7

Red (635nm) laser (3 detectors): AF700, APC or AF647, and APC-Cy7

Storm enables sampling only from 5 mL FACS tubes.

Follow link to see detailed LSRFortessa (Storm) lasers and filter configurations.

Follow link for assistance building an experimental panel for use on the Fortessa (Storm) using FluoroFinder: https://app.fluorofinder.com/pavir