Flow Cytometry Core – Flow Cytometer Information

Flow Cytometer Information

  • Availability: 24 hours, 7 days a week for verified users.
  • Normal Working Hours with Assistance and Flow Cytometer Set Up: Monday- Friday 8:30am-5pm.
  • All benchtop BD Flow Cytometers are located at the address below:

Palo Alto VA
3801 Miranda Ave, Palo Alto
Bldg.101, 4th Floor, Rm. B4-115

Contact for training and/or access to the flow cytometers
Primary Contact: Corey Cain, Ph.D.
Phone: (209) 324-9540
Email: ccain@pavir.org


LSRII (Nickname: Nightcrawler)

BD Serial # S/N H1014

LSR2-Night-CrawlerThe LSRII is a flow cytometer equipped with four lasers (405nm (violet), 488nm (blue), 532nm (yellow-green) and 633nm (red)) and can detect up to 16 fluorophores: FITC, PerCP or PerCPCy5.5, PE, PE-TxRed, PECy5, PECy5.5, PECy7, Pacific Blue, AmCyan OR V500, BV or Qdot565, BV or Qdot605, BV or Qdot655, BV 710, AF700, APC or AF647, and APC-Cy7).  The LSRII has the hardware for automated data acquisition from 96 well plates using the High Throughput Sampler (HTS) system.

Follow link to see detailed LSRII (Nightcrawler) lasers and filter configurations.


LSRFortessa (Nickname: Storm)

BD Serial # H5B500011

LSRFortessa-Storm

The LSRFortessa is a flow cytometer equipped with five lasers (355nm (near UV), 405nm (violet), 488nm (blue), 532nm (yellow-green) and 640nm (red)) and can detect up to 18 fluorophores. Common fluorophores include: BUV, BUV,FITC, PerCP or PerCPCy5.5, PE, PE-TxRed, PECy5, PECy5.5, PECy7, Pacific Blue, AmCyan or V500, BV or Qdot565, BV or Qdot605, BV or Qdot655, BV710, AF700, APC or AF647, and APC-Cy7.

Follow link to see detailed LSRFortessa (Storm) lasers and filter configurations.